Schuurman R, van Strien A, van Steenis B, van der Noordaa J, Sol C
Department of Virology, University of Amsterdam, Academic Medical Centre, The Netherlands.
J Gen Virol. 1992 Nov;73 ( Pt 11):2871-8. doi: 10.1099/0022-1317-73-11-2871.
The early region of bovine polyomavirus (BPyV) was tested for its cell transformation potential employing an assay of dense focus formation. Dense foci of morphologically transformed cells were observed upon transfection of primary rodent cells with a plasmid construct encoding the complete early region of BPyV under the transcriptional control of the long terminal repeat of Rous sarcoma virus. No transformation of primary rodent cells was observed upon transfection of these cells with a plasmid encoding the complete early region of BPyV under the control of its own transcriptional regulatory sequences. In BPyV-transformed cells, the viral sequences had become integrated into the cellular genome, and expression of large T antigen could be detected in a high percentage of cells. The transformed cells were demonstrated to be capable of anchorage-independent growth and to be oncogenic in immunocompromised newborn rats. Therefore BPyV should be considered as a potentially tumorigenic polyomavirus. Since many commercial batches of calf serum have been shown to be contaminated with BPyV, our observations may have implications for the use of calf serum in cell culture.
利用致密集落形成试验检测牛多瘤病毒（BPyV）早期区域的细胞转化潜能。在用编码BPyV完整早期区域的质粒构建体转染原代啮齿动物细胞后，在劳斯肉瘤病毒长末端重复序列的转录控制下，观察到形态转化细胞的致密集落。在用在其自身转录调控序列控制下编码BPyV完整早期区域的质粒转染这些细胞后，未观察到原代啮齿动物细胞的转化。在BPyV转化的细胞中，病毒序列已整合到细胞基因组中，并且在高比例的细胞中可检测到大T抗原的表达。已证明转化细胞能够在不依赖贴壁的情况下生长，并且在免疫受损的新生大鼠中具有致癌性。因此，BPyV应被视为一种潜在的致瘤多瘤病毒。由于已证明许多商业批次的小牛血清被BPyV污染，我们的观察结果可能对细胞培养中小牛血清的使用有影响。
