Moss D, Fargion S, Fracanzani A L, Levi S, Cappellini M D, Arosio P, Powell L W, Halliday J W
Liver Unit, Queensland Institute of Medical Research, Brisbane, Australia.
J Inorg Biochem. 1992;47(3-4):219-27. doi: 10.1016/0162-0134(92)84067-w.
Ferritin receptors are present on the membranes of many normal and malignant cells. The binding specificity of these receptors for H and L subunits was examined using recombinant human ferritin homopolymers. At least two different types of ferritin receptors were found, one derived from normal rat, pig, and human liver which shows similar binding of H- and L-ferritin. The second receptor type, specific for the H-chain ferritin, has been identified on membranes of hepatic and other transformed cells, and of normal lymphoblasts and erythroid precursors. These two receptor types may have different metabolic functions: the hepatic receptor acting as a scavenger for circulating ferritin and possibly for iron exchange between hepatocytes and macrophages; the H-ferritin receptor having a regulatory role which is not directly related to iron metabolism. The expression of the H-ferritin receptor is closely related to the activation and proliferation state of the cells. Addition of H-ferritin to the culture medium of cells expressing the H-ferritin receptor resulted in inhibition of cell proliferation and of colony formation.
铁蛋白受体存在于许多正常细胞和恶性细胞的膜上。使用重组人铁蛋白同聚物研究了这些受体对H亚基和L亚基的结合特异性。发现至少有两种不同类型的铁蛋白受体，一种来源于正常大鼠、猪和人的肝脏，对H型和L型铁蛋白显示出相似的结合能力。第二种受体类型对H链铁蛋白具有特异性，已在肝和其他转化细胞以及正常淋巴母细胞和红系前体细胞的膜上得到鉴定。这两种受体类型可能具有不同的代谢功能：肝脏受体作为循环铁蛋白的清除剂，可能参与肝细胞与巨噬细胞之间的铁交换；H铁蛋白受体具有与铁代谢无直接关系的调节作用。H铁蛋白受体的表达与细胞的激活和增殖状态密切相关。向表达H铁蛋白受体的细胞培养基中添加H铁蛋白会导致细胞增殖和集落形成受到抑制。
