Dosaka-Akita H, Harada M, Miyamoto H, Kawakami Y
First Department of Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1992 Aug;30(8):1441-7.
The clinical importance of ras oncogene product p21 was evaluated in surgically treated non-small cell lung cancer patients. Paraffin sections of tumors were analysed immunohistochemically using anti-ras p21 monoclonal antibody rp35. The ras p21 expression was correlated with clinicopathological parameters and survival. Survival analysis demonstrated significantly longer survival times in patients with p21-negative tumors than those with p21-positive tumors. In Cox's multivariate analysis, ras p21 expression was a major and independent prognostic determinant of survival. On the other hand, in small cell lung cancer, L-myc gene is known to be frequently amplified and overexpressed. Immunoprecipitation analysis of two small cell lung cancer cell lines (classic type) revealed three major L-myc proteins (p60, p66 and p68), all of which were derived from extensive phosphorylation of a p59 protein. Expression and phosphorylation of L-myc protein, as well as the autocrine growth mechanism of gastrin-releasing peptide (GRP), is thought to be involved in the malignant behavior of small cell lung cancer.
在接受手术治疗的非小细胞肺癌患者中评估了ras癌基因产物p21的临床重要性。使用抗ras p21单克隆抗体rp35对肿瘤石蜡切片进行免疫组织化学分析。ras p21表达与临床病理参数及生存率相关。生存分析显示，p21阴性肿瘤患者的生存时间显著长于p21阳性肿瘤患者。在Cox多因素分析中，ras p21表达是生存的主要且独立的预后决定因素。另一方面，在小细胞肺癌中，已知L-myc基因经常扩增和过度表达。对两种小细胞肺癌细胞系（经典型）的免疫沉淀分析显示有三种主要的L-myc蛋白（p60、p66和p68），所有这些蛋白均源自p59蛋白的广泛磷酸化。L-myc蛋白的表达和磷酸化以及胃泌素释放肽（GRP）的自分泌生长机制被认为与小细胞肺癌的恶性行为有关。
