Iwamuro S, Hayashi H, Delbende C, Vaudry H, Kikuyama S
Department of Biology, School of Education, Waseda University, Tokyo, Japan.
Peptides. 1992 Jul-Aug;13(4):729-35. doi: 10.1016/0196-9781(92)90179-7.
The joining peptide (JP) and the N-terminal peptide of proopiomelanocortin (NPP) were isolated from an acid-acetone extract of the distal lobe of the pituitary of the bullfrog, Rana catesbeiana, and purified by gel filtration and reverse-phase high performance liquid chromatography. The amino acid sequence of the bullfrog JP resembled the sequences of the JPs of Rana ridibunda (86% similarity) and Xenopus laevis (54% similarity), as deduced from the nucleotide sequences of their cDNAs. The amino acid sequence of bullfrog NPP showed 100%, 85%, and 50% similarity with those of Rana ridibunda, Xenopus laevis, and human NPPs, respectively. Administration of bullfrog NPP (0.05-5 micrograms/ml) to perifused Rana ridibunda interrenal slices induced a dose-dependent stimulation of corticosterone and aldosterone release. The present results indicate that the primary structure of NPP has been highly conserved during evolution. These data also reveal that NPP, which has no sequence homology with ACTH, exhibits a substantial corticotropic activity.
从牛蛙（Rana catesbeiana）垂体远侧叶的酸 - 丙酮提取物中分离出连接肽（JP）和阿黑皮素原（proopiomelanocortin，POMC）的N端肽（NPP），并通过凝胶过滤和反相高效液相色谱法进行纯化。从牛蛙JP的cDNA核苷酸序列推导得出，其氨基酸序列与食用蛙（Rana ridibunda）的JP序列相似性为86%，与非洲爪蟾（Xenopus laevis）的JP序列相似性为54%。牛蛙NPP的氨基酸序列与食用蛙、非洲爪蟾和人NPP的氨基酸序列相似性分别为100%、85%和50%。向灌注的食用蛙肾上腺切片中给予牛蛙NPP（0.05 - 5微克/毫升）可诱导皮质酮和醛固酮释放呈剂量依赖性刺激。目前的结果表明，NPP的一级结构在进化过程中高度保守。这些数据还表明，与促肾上腺皮质激素（ACTH）无序列同源性的NPP具有显著的促肾上腺皮质活性。
