Rösen-Wolff A, Frank S, Raab K, Moyal M, Becker Y, Darai G
Institut für Medizinische Virologie, Universität Heidelberg, Germany.
Virus Res. 1992 Sep 15;25(3):189-99. doi: 10.1016/0168-1702(92)90133-t.
Herpes simplex virus type 1 (HSV-1) strain HFEM acquired an apathogenic phenotype due to a deletion within the DNA sequences of the BamHI DNA fragment B of the viral genome. In order to investigate the coding strategy of this particular region of the genome of HSV-1 strain HFEM the DNA nucleotide sequence of the BamHI DNA fragment B was determined. This analysis revealed that the BamHI DNA fragment B of HSV-1 strain HFEM comprises 6593 bp, corresponding to the nucleotide positions (np) 113322 to 117088 and np 120643 to 123465 of the genome of HSV-1 strain 17. According to these data the deletion of the genome of HSV-1 strain HFEM occurred between the np 117089 and 120642. The promoter region of the UL56 gene of HSV-1 strain HFEM is a part of the deleted DNA sequences. Therefore, this gene of HSV-1 strain HFEM is affected and cannot be expressed. The first 35 amino acid (AA) residues of the deduced amino acid sequence of the UL56 open reading frame (ORF) were found to be identical to the amino acid sequence of the UL56 genes of HSV-1 strains 17 and F. However, due to a deletion at np 3494 of the BamHI DNA fragment B of HSV-1 strain HFEM the amino acid composition of the predicted UL56 gene of HSV-1 strain HFEM is different from HSV-1 strain 17 between amino acid positions 36 and 233. In addition the deduced amino acid sequence of the IRL (inverted repeat of the long segment) copy of the IE110 gene of HSV-1 strain HFEM was found to be about 342 amino acids shorter than the amino acid sequence of IE110 gene of HSV-1 strain 17 (775 AA). This was based on a point mutation which was detected within the DNA sequences of Exon 3 of this copy of IE110 gene of HSV-1 strain HFEM.
单纯疱疹病毒1型（HSV-1）HFEM毒株由于病毒基因组BamHI DNA片段B的DNA序列内发生缺失而获得了无致病性表型。为了研究HSV-1 HFEM毒株基因组这一特定区域的编码策略，测定了BamHI DNA片段B的DNA核苷酸序列。该分析表明，HSV-1 HFEM毒株的BamHI DNA片段B包含6593 bp，对应于HSV-1 17毒株基因组的核苷酸位置（np）113322至117088以及np 120643至123465。根据这些数据，HSV-1 HFEM毒株基因组的缺失发生在np 117089和120642之间。HSV-1 HFEM毒株UL56基因的启动子区域是缺失的DNA序列的一部分。因此，HSV-1 HFEM毒株的该基因受到影响且无法表达。UL56开放阅读框（ORF）推导的氨基酸序列的前35个氨基酸（AA）残基被发现与HSV-1 17和F毒株的UL56基因的氨基酸序列相同。然而，由于HSV-1 HFEM毒株BamHI DNA片段B的np 3494处发生缺失，HSV-1 HFEM毒株预测的UL56基因的氨基酸组成在氨基酸位置36至233之间与HSV-1 17毒株不同。此外，发现HSV-1 HFEM毒株IE110基因的IRL（长片段反向重复）拷贝推导的氨基酸序列比HSV-1 17毒株IE110基因的氨基酸序列（775个AA）短约342个氨基酸。这是基于在HSV-1 HFEM毒株该IE110基因拷贝的外显子3的DNA序列中检测到的一个点突变。
