Moyal M, Raibstein I, Rösen A, Darai G, Becker Y
Department of Molecular Virology, Faculty of Medicine, Hebrew University of Jerusalem, Israel.
Virus Genes. 1988 Mar;1(2):165-74. doi: 10.1007/BF00555935.
The restriction cleavage sites of the BamHI-B and BamHI-E DNA fragments of several Herpes simplex virus type 1 (HVS-1) strains were mapped. These fragments are situated at the ends of the long unique regions and share homologous sequences in the repeat components (TRL and IRL) of the genome. All the strains analyzed were found to have deletions in the Hpal-P fragment, situated in the BamHI-B fragment. Five strains were further analyzed and the deletions were located in the Smal-A fragment (within the Hpal-P fragment). The BamHI-E fragment of four recombinants (obtained by recombination between the HFEM genome and the BamHI-B fragment or part of it from the HSV-1 F strain) were almost identical but differed from another strain [NIH(LP)]. Comparison of the BamHI-B and the BamHI-E fragments of the same strain revealed that the fragments were not identical in all cases.
对几株单纯疱疹病毒1型（HSV-1）菌株的BamHI-B和BamHI-E DNA片段的限制性酶切位点进行了定位。这些片段位于长独特区域的末端，并且在基因组的重复组分（TRL和IRL）中具有同源序列。发现所有分析的菌株在位于BamHI-B片段中的HpaI-P片段中存在缺失。对五株菌株进行了进一步分析，缺失位于SmaI-A片段（在HpaI-P片段内）。四个重组体（通过HFEM基因组与HSV-1 F菌株的BamHI-B片段或其部分之间的重组获得）的BamHI-E片段几乎相同，但与另一菌株[NIH(LP)]不同。同一菌株的BamHI-B和BamHI-E片段的比较表明，这些片段并非在所有情况下都相同。
