Kasüske A, Wedler H, Schulze S, Becher D
Ernst-Moritz-Arndt-Universität Greifswald, Fachrichtung Biologie, Institut für Genetik, Germany.
Yeast. 1992 Sep;8(9):691-7. doi: 10.1002/yea.320080902.
Conditions for efficient and quick transformation by electroporation were developed in Candida maltosa. To investigate the efficiency of transformation with integrative as well as with autonomously replicating plasmids, a series of vectors was constructed for homologous transformation of this species. Transformants were obtained with different plasmids as covalently closed circular molecules and as linearized DNA. The influence of recipient strain and plasmid type as well as of cell number and parameters of the supplied electrical pulse on the transformation efficiency have been investigated. A maximum of 7000 transformants per 100 ng of plasmid DNA was reached. The efficiency of transformation was compared with that of the LiCl method.
在麦芽糖假丝酵母中建立了通过电穿孔进行高效快速转化的条件。为了研究整合型质粒和自主复制型质粒的转化效率，构建了一系列载体用于该物种的同源转化。以共价闭合环状分子和线性化DNA等不同质粒获得了转化子。研究了受体菌株、质粒类型以及细胞数量和所提供电脉冲参数对转化效率的影响。每100 ng质粒DNA最多可获得7000个转化子。将转化效率与LiCl法进行了比较。
