一个自交不亲和油菜品系中的S位点受体激酶基因编码一种功能性丝氨酸/苏氨酸激酶。
The S-locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase.
作者信息
Goring D R, Rothstein S J
机构信息
Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
出版信息
Plant Cell. 1992 Oct;4(10):1273-81. doi: 10.1105/tpc.4.10.1273.
Abstract
An S-receptor kinase (SRK) cDNA, SRK-910, from the active S-locus in a self-incompatible Brassica napus W1 line has been isolated and characterized. The SRK-910 gene is predominantly expressed in pistils and segregates with the W1 self-incompatibility phenotype in an F2 population derived from a cross between the self-incompatible W1 line and a self-compatible Westar line. Analysis of the predicted amino acid sequence demonstrated that the extracellular receptor domain is highly homologous to S-locus glycoproteins, whereas the cytoplasmic kinase domain contains conserved amino acids present in serine/threonine kinases. An SRK-910 kinase protein fusion was produced in Escherichia coli and found to contain kinase activity. Phosphoamino acid analysis confirmed that only serine and threonine residues were phosphorylated. Thus, the SRK-910 gene encodes a functional serine/threonine receptor kinase.
摘要
从自交不亲和的甘蓝型油菜W1系的活性S位点分离并鉴定了一个S受体激酶（SRK）cDNA，即SRK - 910。SRK - 910基因主要在雌蕊中表达，并且在由自交不亲和的W1系与自交亲和的Westar系杂交产生的F2群体中，与W1自交不亲和表型共分离。对预测的氨基酸序列分析表明，细胞外受体结构域与S位点糖蛋白高度同源，而细胞质激酶结构域含有丝氨酸/苏氨酸激酶中存在的保守氨基酸。在大肠杆菌中产生了SRK - 910激酶蛋白融合体，并发现其具有激酶活性。磷酸氨基酸分析证实只有丝氨酸和苏氨酸残基被磷酸化。因此，SRK - 910基因编码一种功能性丝氨酸/苏氨酸受体激酶。
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