1State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, 100091 Beijing, China.
2Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, 100091 Beijing, China.
Commun Biol. 2019 Jun 18;2:215. doi: 10.1038/s42003-019-0474-7. eCollection 2019.
The genus comprises a complex amalgam of ancient and modern species that has become a prime model for evolutionary and taxonomic studies. Here we sequenced the genomes of 10 species from five sections of the genus , identified 71 million genomic variations, and observed new correlations between the single-nucleotide polymorphism-structural variation (SNP-SV) density and indel-SV density to complement the SNP-indel density correlation reported in mammals. Disease resistance genes (R genes) with heterozygous loss-of-function (LOF) were significantly enriched in the 10 species, which increased the diversity of poplar R genes during evolution. Heterozygous LOF mutations in the self-incompatibility genes were closely related to the self-fertilization of poplar, suggestive of genomic control of self-fertilization in dioecious plants. The phylogenetic genome-wide SNPs tree also showed possible ancient hybridization among species in sections Tacamahaca, Aigeiros, and Leucoides. The pangenome resource also provided information for poplar genetics and breeding.
该属包含了古老和现代物种的复杂混合体，已成为进化和分类学研究的主要模式。在这里，我们对该属五个部分的 10 个物种进行了基因组测序，鉴定出了 7100 万个基因组变异，并观察到单核苷酸多态性-结构变异（SNP-SV）密度与插入缺失-SV 密度之间的新关联，以补充哺乳动物中报道的 SNP-插入缺失密度关联。具有杂合功能丧失（LOF）的抗病基因（R 基因）在 10 个物种中显著富集，这增加了杨树 R 基因在进化过程中的多样性。自交不亲和基因中的杂合 LOF 突变与杨树的自交密切相关，表明雌雄异株植物的基因组对自交的控制。系统发育全基因组 SNP 树还显示出 Tacamahaca、Aigeiros 和 Leucoides 等部分的物种之间可能存在古老的杂交。泛基因组资源还为杨树遗传学和育种提供了信息。
