一种植物类受体基因，即甘蓝的S位点受体激酶，编码一种功能性丝氨酸/苏氨酸激酶。
A plant receptor-like gene, the S-locus receptor kinase of Brassica oleracea L., encodes a functional serine/threonine kinase.
作者信息
Stein J C, Nasrallah J B
机构信息
Division of Biological Sciences, Cornell University, Ithaca, New York 14853.
出版信息
Plant Physiol. 1993 Mar;101(3):1103-6. doi: 10.1104/pp.101.3.1103.
Abstract
To investigate the catalytic properties of the Brassica oleracea S-locus receptor kinase (SRK), we have expressed the domain that is homologous to protein kinases as a fusion protein in Escherichia coli. Following in vivo labeling of cultures with 32P-labeled inorganic phosphate, we observed phosphorylation of the fusion protein on serine and threonine, but not on tyrosine. In contrast, labeling was not observed when lysine-524, a residue conserved among all protein kinases, was mutated to arginine, thus confirming that SRK phosphorylation was the result of intrinsic serine/threonine kinase activity.
摘要
为了研究甘蓝（Brassica oleracea）S位点受体激酶（SRK）的催化特性，我们已将与蛋白激酶同源的结构域作为融合蛋白在大肠杆菌中表达。在用32P标记的无机磷酸盐对培养物进行体内标记后，我们观察到融合蛋白在丝氨酸和苏氨酸上发生磷酸化，但在酪氨酸上未发生磷酸化。相比之下，当所有蛋白激酶中保守的赖氨酸-524突变为精氨酸时，未观察到标记，从而证实SRK磷酸化是内在丝氨酸/苏氨酸激酶活性的结果。
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