Chen C W, Yu T W, Chung H M, Chou C F
Institute of Genetics, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
J Bacteriol. 1992 Dec;174(23):7762-9. doi: 10.1128/jb.174.23.7762-7769.1992.
Transposition of a new 5.4-kb transposon, Tn4811, of Streptomyces lividans to the melC operon of Streptomyces antibioticus on plasmid pIJ702 was discovered. The nucleotide sequence of this copy of Tn4811, which contained an imperfect (9 of 11 bp) terminal inverted repeat, five putative Streptomyces coding sequences for an oxidoreductase and its transcription regulator, and three transposition-related proteins, was determined. SLP- strains of S. lividans contained one copy (A) of Tn4811, while SLP2+ strains contained an additional copy (B) on the SLP2 plasmid. The nucleotide sequences at three insertion junctions of Tn4811 were determined. Copy B lacked 41 bp from the left end. At the other five junctions the duplication of a putative 3-bp target sequence (TGA) was observed. A sequence of less than 3 kb homologous to Tn4811 was present in S. antibioticus. DNA homologous to Tn4811 was not detected in 14 other Streptomyces species.
发现了天蓝色链霉菌的一个新的5.4千碱基转座子Tn4811转座到质粒pIJ702上的抗生链霉菌的melC操纵子上。测定了这个Tn4811拷贝的核苷酸序列，它包含一个不完全的（11个碱基中的9个）末端反向重复序列、五个推测的链霉菌编码序列，分别编码一种氧化还原酶及其转录调节因子，以及三种与转座相关的蛋白质。天蓝色链霉菌的SLP-菌株含有一个Tn4811拷贝（A），而SLP2+菌株在SLP2质粒上还含有一个额外的拷贝（B）。测定了Tn4811三个插入位点的核苷酸序列。拷贝B从左端缺失41个碱基。在其他五个位点观察到一个推测的3碱基靶序列（TGA）的重复。抗生链霉菌中存在一段与Tn4811同源的不到3千碱基的序列。在其他14种链霉菌中未检测到与Tn4811同源的DNA。
