Tully M, Sharpe S A, Ashworth S
Division of Pathology, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.
Res Microbiol. 1992 Jun;143(5):471-9. doi: 10.1016/0923-2508(92)90093-4.
Two of three highly virulent strains of Legionella pneumophila could act as recipients at high frequencies in conjugation experiments with Escherichia coli donor strains carrying broad host-range plasmids belonging to incompatibility groups N, P and W. All broad host-range and most transposon-delivery plasmids persisted within transconjugants with high stability. Only one (pSUP1021) of several vehicles designed for the delivery of transposons into the chromosome of Gram-negative bacteria was found to yield transposon mutants of Legionella at a detectable frequency.
