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嗜肺军团菌中的转座子诱变。II. —— 在培养的巨噬细胞内生长受损且体内毒力降低的突变体。

Transposon mutagenesis in Legionella pneumophila. II.--Mutants exhibiting impaired intracellular growth within cultured macrophages and reduced virulence in vivo.

作者信息

Tully M, Williams A, Fitzgeorge R B

机构信息

Division of Pathology, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.

出版信息

Res Microbiol. 1992 Jun;143(5):481-8. doi: 10.1016/0923-2508(92)90094-5.

DOI:10.1016/0923-2508(92)90094-5
PMID:1333092
Abstract

Transposon Tn5 mutants of L. pneumophila were isolated and screened for loss of virulence-associated characteristics. Three mutants were found with normal ability to produce putative pathogenicity determinants and to be endocytosed by guinea pig alveolar macrophages in vitro but with a greatly reduced ability to multiply within them. These mutants showed considerable loss of virulence in an authentic animal model of the pneumonia.

摘要

分离出嗜肺军团菌的转座子Tn5突变体，并筛选其与毒力相关特征的丧失情况。发现三个突变体在体外产生假定致病性决定因素以及被豚鼠肺泡巨噬细胞内吞的能力正常，但在细胞内繁殖的能力大大降低。在真实的肺炎动物模型中，这些突变体显示出相当大的毒力丧失。

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Transposon mutagenesis in Legionella pneumophila. II.--Mutants exhibiting impaired intracellular growth within cultured macrophages and reduced virulence in vivo.嗜肺军团菌中的转座子诱变。II. —— 在培养的巨噬细胞内生长受损且体内毒力降低的突变体。
Res Microbiol. 1992 Jun;143(5):481-8. doi: 10.1016/0923-2508(92)90094-5.
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The Legionella pneumophila icm locus: a set of genes required for intracellular multiplication in human macrophages.嗜肺军团菌icm基因座：一组人类巨噬细胞内增殖所需的基因。
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Mutagenesis of Legionella pneumophila using Tn903 dlllacZ: identification of a growth-phase-regulated pigmentation gene.利用Tn903 dlllacZ对嗜肺军团菌进行诱变：鉴定一个生长阶段调控的色素沉着基因。
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引用本文的文献

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Subinhibitory concentrations of antimicrobial agents reduce the uptake of Legionella pneumophila into Acanthamoeba castellanii and U937 cells by altering the expression of virulence-associated antigens.抗菌剂的亚抑制浓度通过改变毒力相关抗原的表达，减少嗜肺军团菌进入卡氏棘阿米巴和U937细胞的摄取。
Antimicrob Agents Chemother. 1998 Nov;42(11):2870-6. doi: 10.1128/AAC.42.11.2870.
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Influence of iron-limited continuous culture on physiology and virulence of Legionella pneumophila.铁限制连续培养对嗜肺军团菌生理特性和毒力的影响。
Infect Immun. 1995 Nov;63(11):4224-30. doi: 10.1128/iai.63.11.4224-4230.1995.