嗜肺军团菌中的转座子诱变。II. —— 在培养的巨噬细胞内生长受损且体内毒力降低的突变体。

Transposon mutagenesis in Legionella pneumophila. II.--Mutants exhibiting impaired intracellular growth within cultured macrophages and reduced virulence in vivo.

作者信息

Tully M, Williams A, Fitzgeorge R B

机构信息

Division of Pathology, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.

出版信息

Res Microbiol. 1992 Jun;143(5):481-8. doi: 10.1016/0923-2508(92)90094-5.

DOI:10.1016/0923-2508(92)90094-5

PMID:1333092

Abstract

Transposon Tn5 mutants of L. pneumophila were isolated and screened for loss of virulence-associated characteristics. Three mutants were found with normal ability to produce putative pathogenicity determinants and to be endocytosed by guinea pig alveolar macrophages in vitro but with a greatly reduced ability to multiply within them. These mutants showed considerable loss of virulence in an authentic animal model of the pneumonia.

摘要

分离出嗜肺军团菌的转座子Tn5突变体，并筛选其与毒力相关特征的丧失情况。发现三个突变体在体外产生假定致病性决定因素以及被豚鼠肺泡巨噬细胞内吞的能力正常，但在细胞内繁殖的能力大大降低。在真实的肺炎动物模型中，这些突变体显示出相当大的毒力丧失。