Tsikas D, Fauler J, Frölich J C
Institute of Clinical Pharmacology, Hannover Medical School, FRG.
Eicosanoids. 1992;5 Suppl:S7-9.
Gas chromatographic-(tandem) mass spectrometric (GC-MS(-MS)) methods for the analysis of cysteinyl leukotrienes (LTs) and leukotriene B4 (LTB4) in biological fluids were developed. The enzymatic synthesis of the stable-isotope labelled analogues [1,1-18O2] LTE4 and [14,15,17,17,18,18-2H6] LTB4 in high isotopic purity is described. Utilizing [1,1-18O2] LTE4 as an internal standard and GC-MS-MS enhanced cysteinyl LTs synthesis was found in patients with multiple trauma and psoriasis compared to healthy volunteers, respectively. The metabolism of LTB4 by human monocytes was investigated and two novel LTB4 metabolites were structurally identified by GC-MS as 10,11-dihydro-LTB4 and 10,11-dihydro-12-oxo-LTB4.
已开发出用于分析生物流体中半胱氨酰白三烯（LTs）和白三烯B4（LTB4）的气相色谱 -（串联）质谱（GC-MS(-MS)）方法。描述了高同位素纯度的稳定同位素标记类似物[1,1-18O2] LTE4和[14,15,17,17,18,18-2H6] LTB4的酶促合成。分别以[1,1-18O2] LTE4作为内标，利用GC-MS-MS发现，与健康志愿者相比，多发伤患者和银屑病患者中半胱氨酰LTs的合成增加。研究了人单核细胞对LTB4的代谢，并通过GC-MS在结构上鉴定出两种新的LTB4代谢物为10,11-二氢-LTB4和10,11-二氢-12-氧代-LTB4。
