Fauler J, Neumann C, Tsikas D, Frölich J
Department of Clinical Pharmacology, Hannover Medical School, Germany.
J Invest Dermatol. 1992 Jul;99(1):8-11. doi: 10.1111/1523-1747.ep12611380.
Cysteinyl leukotriene synthesis was investigated in patients with psoriasis. A non-invasive test requiring no stimulation was employed by measuring the major index metabolite of LTC4, which appears in urine. The presence of this metabolite, LTE4, was shown unequivocally by gas chromatography-mass spectrometry. Routinely LTE4 was quantitated by specific radio immunoassay after its isolation by reversed-phase high-performance liquid chromatography. Furthermore, in representative samples amounts of LTE4 obtained by radioimmunoassay were validated by gas chromatography-mass spectrometry. We demonstrate a significant (p less than 0.01) more than fourfold increase of urinary LTE4 in psoriasis compared to healthy volunteers. Urinary LTE4 was log normally distributed with geometric mean values (95% confidence intervals) of 11 (9-14) nmol LTE4/mol creatinine in healthy volunteers (n = 11) and 51 (28-95) nmol LTE4/mol creatinine in psoriasis (n = 9). The present study shows that cysteinyl leukotriene synthesis is enhanced in patients with psoriasis and that measurement of urinary LTE4 is a useful parameter to monitor its rate of synthesis.
对银屑病患者的半胱氨酰白三烯合成进行了研究。采用了一种无需刺激的非侵入性检测方法，通过测量尿液中出现的LTC4主要指标代谢物来进行。气相色谱 - 质谱法明确显示了这种代谢物LTE4的存在。常规情况下，LTE4在通过反相高效液相色谱分离后，通过特异性放射免疫测定法定量。此外，在代表性样本中，通过放射免疫测定法获得的LTE4量通过气相色谱 - 质谱法进行了验证。我们证明，与健康志愿者相比，银屑病患者尿液中LTE4显著增加（p小于0.01），增加了四倍多。尿液LTE4呈对数正态分布，健康志愿者（n = 11）的几何平均值（95%置信区间）为11（9 - 14）nmol LTE4/摩尔肌酐，银屑病患者（n = 9）为51（28 - 95）nmol LTE4/摩尔肌酐。本研究表明，银屑病患者的半胱氨酰白三烯合成增强，尿液LTE4的测量是监测其合成速率的有用参数。
