可变α-生育酚对未转化和转化成纤维细胞中谷胱甘肽过氧化物酶活性的刺激及保护作用

Variable alpha-tocopherol stimulation and protection of glutathione peroxidase activity in non-transformed and transformed fibroblasts.

作者信息

Goldring C E, Hu W L, Rao N R, Rice-Evans C, Burdon R H, Diplock A T

机构信息

Division of Biochemistry, UMDS, Guy's Hospital, London, U.K.

出版信息

EXS. 1992;62:251-6. doi: 10.1007/978-3-0348-7460-1_26.

DOI:10.1007/978-3-0348-7460-1_26

PMID:1333309

Abstract

Studies on glutathione metabolism in an established baby hamster kidney cell line (BHK-21/C13) and in its polyoma virus-transformed counterpart (BHK-21/PyY), have revealed a significant stimulation of intracellular glutathione peroxidase activity (Se-independent plus Se-dependent) by alpha-tocopherol supplementation (14 microM). This stimulation was found to be much greater in the transformed cells. Other GSH-requiring enzyme activities (namely glutathione reductase and glutathione transferase) were unaltered by alpha-tocopherol treatment, suggesting a degree of specificity in its action on GSHpx. In unsupplemented growth media, the GSHpx activity in both cell lines was significantly decreased by an oxidative stress. However, the same stress applied to the alpha-tocopherol-supplemented cells had no effect on the stimulated GSHpx activity, suggesting a protection afforded by the alpha-tocopherol.

摘要

对已建立的幼仓鼠肾细胞系（BHK - 21/C13）及其多瘤病毒转化的对应细胞系（BHK - 21/PyY）中的谷胱甘肽代谢进行的研究表明，通过补充α - 生育酚（14微摩尔）可显著刺激细胞内谷胱甘肽过氧化物酶活性（硒非依赖性加硒依赖性）。在转化细胞中发现这种刺激作用要大得多。其他需要谷胱甘肽的酶活性（即谷胱甘肽还原酶和谷胱甘肽转移酶）不受α - 生育酚处理的影响，这表明其对谷胱甘肽过氧化物酶的作用具有一定程度的特异性。在未补充的生长培养基中，氧化应激会使两种细胞系中的谷胱甘肽过氧化物酶活性显著降低。然而，对补充了α - 生育酚的细胞施加相同的应激对受刺激的谷胱甘肽过氧化物酶活性没有影响，这表明α - 生育酚提供了保护作用。