补充硒和维生素E：人体组织中谷胱甘肽过氧化物酶的活性

Selenium and vitamin E supplementation: activities of glutathione peroxidase in human tissues.

作者信息

Thomson C D, Steven S M, van Rij A M, Wade C R, Robinson M F

机构信息

Department of Human Nutrition, University of Otago, Dunedin, New Zealand.

出版信息

Am J Clin Nutr. 1988 Aug;48(2):316-23. doi: 10.1093/ajcn/48.2.316.

DOI:10.1093/ajcn/48.2.316

PMID:3407611

Abstract

Twenty-seven New Zealand women received daily for 4 wk, 200 micrograms selenium as sodium selenite, 170 mg alpha-tocopherol acetate, or a placebo. Se supplementation raised platelet selenoglutathione peroxidase (Se-GSHPx, p less than 0.001) and also Se and Se-GSHPx in whole blood and plasma. Se concentrations and Se-GSHPx activities in liver biopsies taken after supplementation were greater (p less than 0.05) for the Se group and a good correlation was found between Se and Se-GSHPx in liver and muscle for all subjects. Platelet Se-GSHPx correlated well with Se and Se-GSHPx in liver, indicating its suitability for assessing Se bioavailability. This is the first reported study of relationships between Se and Se-GSHPx in human liver and muscle tissue and platelet Se-GSHPx after Se supplementation. These observations verify in man relationships observe in animal studies, giving support to assumptions made in methods for assessing Se status and bioavailability in man, in particular the use of platelet GSHPx.

摘要

27名新西兰女性连续4周每天服用200微克亚硒酸钠形式的硒、170毫克醋酸生育酚或安慰剂。补充硒可提高血小板硒谷胱甘肽过氧化物酶（Se-GSHPx，p<0.001），同时也提高全血和血浆中的硒及Se-GSHPx。补充后采集的肝脏活检样本中，硒组的硒浓度和Se-GSHPx活性更高（p<0.05），并且在所有受试者中，肝脏和肌肉中的硒与Se-GSHPx之间存在良好的相关性。血小板Se-GSHPx与肝脏中的硒和Se-GSHPx相关性良好，表明其适用于评估硒的生物利用度。这是首次报道的关于补充硒后人体肝脏和肌肉组织中硒与Se-GSHPx以及血小板Se-GSHPx之间关系的研究。这些观察结果证实了在动物研究中观察到的人体中的关系，支持了在评估人体硒状态和生物利用度方法中所做的假设，特别是血小板谷胱甘肽过氧化物酶的应用。