Schmidt C A, Oettle H, Neubauer A, Seeger K, Thiel E, Huhn D, Siegert W, Ludwig W D
Abt. Hämatologie/Onkologie, Klinikum Rudolf Virchow, Freie Universität Berlin, Germany.
Leukemia. 1992 Dec;6(12):1263-7.
In order to investigate the role of T-cell receptor (TcR)-delta and TcR-gamma gene rearrangements and/or deletions in acute myeloid leukemia (AML) coexpressing T-cell-associated antigens (i.e. CD2 and/or CD4 and/or CD7), we examined blasts from a selected group of 56 AML cases (25 children, 31 adults) coexpressing either of these antigens without cytoplasmic CD3 expression. Forty-four typical AML cases (7 children, 37 adults) without T-cell associated antigens were further studied as controls. Germline configuration of the TcR-delta gene was observed in 91 out of the total of 100 AML cases investigated. Eight of nine cases with rearranged or deleted TcR-delta genes coexpressed T-cell-associated antigens. Blast cells of 7/9 cases were classified as FAB M1, two as FAB M2. In six of these cases TcR-gamma gene rearrangements were also detected. TcR-delta alterations were predominantly found in children whose blasts coexpressed T-lymphoid associated antigens (6/25, 24%), but were rarely detected in adult AML with or without coexpression of T-cell antigens (2/31 and 0/37, respectively).
为了研究T细胞受体（TcR）-δ和TcR-γ基因重排及/或缺失在共表达T细胞相关抗原（即CD2和/或CD4和/或CD7）的急性髓系白血病（AML）中的作用，我们检测了一组选定的56例AML病例（25例儿童，31例成人）的原始细胞，这些病例共表达上述任何一种抗原且无细胞质CD3表达。另外选取44例无T细胞相关抗原的典型AML病例（7例儿童，37例成人）作为对照进行进一步研究。在所研究的100例AML病例中，共91例观察到TcR-δ基因的种系构型。9例TcR-δ基因重排或缺失的病例中有8例共表达T细胞相关抗原。9例中的7例原始细胞被分类为FAB M1，2例为FAB M2。其中6例还检测到TcR-γ基因重排。TcR-δ改变主要见于原始细胞共表达T淋巴细胞相关抗原的儿童（6/25，24%），而在有或无T细胞抗原共表达的成人AML中很少检测到（分别为2/31和0/37）。
