Tong J H, Dong S, Chen Y, Qian Z Z, Gu L J, Zhang Y M, Wang Z Y, Chen S J, Chen Z
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University.
Chin Med J (Engl). 1994 Jan;107(1):12-8.
The immunophenotype, rearrangements of T cell receptor (TCR) gamma and delta chain genes as well as the immunoglobulin heavy chain (IgH) gene were studied in 37 cases of morphologically defined acute lymphoblastic leukemia (ALL). According to the expression of differentiation antigens, 8 cases were classified as T-ALL, 26 B lineage ALL, 2 acute undifferentiated leukemia (AUL) and myeloid phenotype. An order of TCR gene rearrangements was observed in T-ALL, with the rearrangement of delta gene preceding that of gamma gene. Both genes were also found frequently rearranged and/or deleted in high proportions of the ALL of B cell lineage. However, the patterns of gene rearrangements were somewhat different between the T and B lineage ALLs. In contrast, the IgH gene rearrangements were observed only in the B lineage ALL. The immunogenotype analysis of ALL proved to be a useful marker of the clonality and provided us with important information on early human lymphoid differentiation. We conclude that the determination of TCR gamma gene V-J junctional sequence can be used as clonal marker for detecting the minimal residual disease during clinical remission.
对37例形态学确诊的急性淋巴细胞白血病（ALL）患者的免疫表型、T细胞受体（TCR）γ和δ链基因重排以及免疫球蛋白重链（IgH）基因进行了研究。根据分化抗原的表达情况，8例被分类为T-ALL，26例为B系ALL，2例为急性未分化白血病（AUL）及髓系表型。在T-ALL中观察到TCR基因重排顺序，δ基因重排在γ基因之前。在高比例的B细胞系ALL中也经常发现这两个基因重排和/或缺失。然而，T系和B系ALL之间的基因重排模式有所不同。相比之下，仅在B系ALL中观察到IgH基因重排。ALL的免疫基因型分析被证明是克隆性的有用标志物，并为我们提供了有关早期人类淋巴细胞分化的重要信息。我们得出结论，TCRγ基因V-J连接序列的测定可作为临床缓解期检测微小残留病的克隆标志物。
