Fung M K, Skinner H B, Bankaitis V A
Department of Cell Biology, University of Alabama, Birmingham 35294.
Curr Opin Genet Dev. 1992 Oct;2(5):775-9. doi: 10.1016/s0959-437x(05)80138-8.
During the past year, a powerful combination of genetic and biochemical approaches has yielded fascinating information with respect to the question of how proteins cross membranes and subsequently traffic between intracellular compartments of the yeast secretory pathway. Fundamental advances have been made in two specific areas. These include experiments that have provided new perspectives with respect to the nature of the soluble machinery involved in facilitating protein traffic from the cytoplasm to the lumen of the endoplasmic reticulum, and work that has provided a biochemical description of what may in effect represent a membranous ligand-gated channel that is required for protein translocation into the endoplasmic reticulum lumen.
在过去的一年里，遗传学和生物化学方法的有力结合，就蛋白质如何跨膜以及随后在酵母分泌途径的细胞内区室之间运输这一问题，产生了引人入胜的信息。在两个特定领域取得了根本性进展。其中包括一些实验，这些实验就促进蛋白质从细胞质运输到内质网腔的可溶性机制的性质提供了新的视角；还有一些工作，对实际上可能代表蛋白质转运到内质网腔所需的膜性配体门控通道进行了生化描述。
