Deshaies R J, Kepes F, Böhni P C
Trends Genet. 1989 Mar;5(3):87-93. doi: 10.1016/0168-9525(89)90032-2.
The earliest events in the export of secretory proteins from eukaryotic cells are their insertion into and transport across the membrane of the endoplasmic reticulum, followed by signal peptide cleavage and transfer of core oligosaccharides to specific asparagine residues. Much has been learned through reconstitution of these processes in vitro using cell-free extracts prepared from mammalian and yeast cells. Now, a combination of genetic, molecular and biochemical approaches are being employed to study the early stages of protein secretion in the yeast Saccharomyces cerevisiae.
真核细胞中分泌蛋白输出的最早事件是它们插入内质网膜并穿过内质网膜，随后信号肽被切割，核心寡糖转移到特定的天冬酰胺残基上。通过使用从哺乳动物和酵母细胞制备的无细胞提取物在体外重建这些过程，人们已经了解了很多信息。现在，遗传、分子和生化方法的结合正被用于研究酿酒酵母中蛋白质分泌的早期阶段。
