Sugiura H, Yamauchi T
Department of Cell Biology, Tokyo Metropolitan Institute for Neuroscience, Japan.
Brain Res. 1992 Oct 9;593(1):97-104. doi: 10.1016/0006-8993(92)91269-k.
Developmental changes in Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) have been immunochemically examined in the forebrain, cerebellum and brainstem of the rat using antibodies against its alpha or beta protein. The concentration of alpha and beta proteins varied markedly in different brain regions at increasing postnatal ages. In early postnatal brain, the concentration of the alpha and beta proteins was low, and a large increase was observed between postnatal days 10 and 30. The maximum expression of the alpha protein was in the order of 6.01, 2.33 and 0.168 micrograms/mg of forebrain, brainstem and cerebellum proteins respectively, in the soluble or particulate fraction. On the other hand, that of the beta protein was in the order of 1.81, 0.495 and 0.291 micrograms/mg of forebrain, cerebellum or brainstem protein. The ratio of alpha and beta proteins also differed in the soluble and particulate fractions. The maximum expression of the alpha protein was observed at day 30 in soluble and particulate fractions of forebrain, and at day 20 in those of the brainstem. The major alpha protein peak was observed on or after day 30 in particulate and soluble fractions from cerebellum, respectively. The maximum expression of the beta protein was observed at day 20 in soluble and particulate fractions of the forebrain as well as in soluble fraction of the cerebellum, and was observed at day 30 in the particulate fraction of cerebellum. The expression of the alpha and beta proteins roughly correlated with the CaM kinase II activity from forebrain and brainstem.
利用针对钙调蛋白依赖蛋白激酶II（CaM激酶II）α或β蛋白的抗体，对大鼠前脑、小脑和脑干中CaM激酶II的发育变化进行了免疫化学检测。随着出生后年龄的增加，α和β蛋白的浓度在不同脑区有显著差异。在出生后早期的大脑中，α和β蛋白的浓度较低，在出生后第10天到第30天之间观察到大幅增加。α蛋白的最大表达量，在可溶性或颗粒部分中，分别以前脑、脑干和小脑蛋白每毫克6.01、2.33和0.168微克的顺序排列。另一方面，β蛋白的最大表达量分别以前脑、小脑或脑干蛋白每毫克1.81、0.495和0.291微克的顺序排列。α和β蛋白的比例在可溶性和颗粒部分也有所不同。α蛋白在出生后第30天在前脑的可溶性和颗粒部分中观察到最大表达，在脑干的相应部分中在出生后第20天观察到最大表达。小脑颗粒和可溶性部分中，主要的α蛋白峰值分别在出生后第30天及之后观察到。β蛋白的最大表达在出生后第20天在前脑的可溶性和颗粒部分以及小脑的可溶性部分中观察到，在小脑颗粒部分中在出生后第30天观察到。α和β蛋白的表达与前脑和脑干中的CaM激酶II活性大致相关。
