Gadelha F R, Hanna P M, Mason R P, Docampo R
Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.
Chem Biol Interact. 1992 Nov 30;85(1):35-48. doi: 10.1016/0009-2797(92)90051-l.
Crystal violet (gentian violet) can undergo an oxidative metabolism, catalyzed by horseradish peroxidase, resulting in formaldehyde formation. The N-demethylation reaction was strongly inhibited by reduced glutathione. Evidence for the formation of a crystal violet radical during the horseradish peroxidase catalyzed reaction was the detection of thiyl and ascorbate radicals from glutathione and ascorbate, respectively. The concentration of radicals from both compounds was significantly increased in the presence of crystal violet. Oxygen uptake was stimulated when glutathione was present in the system and this oxygen uptake was dependent on the dye and enzyme concentration. Oxygen uptake did not occur when ascorbate, instead of glutathione, was present in the system. However, when glutathione was present, ascorbate totally inhibited the glutathione-stimulated oxygen uptake in the crystal violet/horseradish peroxidase/hydrogen peroxide system. Although a weak ESR spectrum from a crystal violet-derived free radical was detected when the dye reacted with H2O2 and horseradish peroxidase, using the fast flow technique, this spectrum could not be interpreted.
结晶紫（龙胆紫）可在辣根过氧化物酶催化下进行氧化代谢，生成甲醛。谷胱甘肽可强烈抑制N - 去甲基化反应。在辣根过氧化物酶催化反应过程中形成结晶紫自由基的证据是，分别从谷胱甘肽和抗坏血酸中检测到硫自由基和抗坏血酸自由基。在结晶紫存在的情况下，这两种化合物产生的自由基浓度显著增加。当体系中存在谷胱甘肽时，氧气吸收受到刺激，且这种氧气吸收依赖于染料和酶的浓度。当体系中存在抗坏血酸而非谷胱甘肽时，未发生氧气吸收。然而，当存在谷胱甘肽时，抗坏血酸在结晶紫/辣根过氧化物酶/过氧化氢体系中完全抑制了谷胱甘肽刺激的氧气吸收。尽管使用快速流动技术，当染料与过氧化氢和辣根过氧化物酶反应时，检测到了来自结晶紫衍生自由基的微弱电子自旋共振光谱，但该光谱无法解析。
