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[为器官移植目的将人淋巴细胞保存在液氮中]

[Preservation of human lymphocytes in liquid nitrogen for the purpose of organ transplantation].

作者信息

Popa I P, Chioreanu M, Mihailov A, Felmeri A

出版信息

Rev Chir Oncol Radiol O R L Oftalmol Stomatol Chir. 1976 Mar-Apr;25(2):133-6.

PMID:133393
Abstract

Repeated typing of organ receptors, indifferent of the technique employed, as well as the preparation of anti-sera of the HL-A type from multiple-pregnancy women (by repeated testing on known lymphocytes panels), suppose the storage for long periods of time of human lymphocytes at low temperatures. The authors present a technique adapted for the storage of lymphocytes in liquid nitrogen allowing for the survival of these cells, in very satisfactory conditions, for long periods of time.

摘要

无论采用何种技术,对器官受体进行重复分型,以及从多胎孕妇中制备HL - A型抗血清(通过在已知淋巴细胞面板上进行反复检测),都需要在低温下长时间保存人类淋巴细胞。作者提出了一种适用于在液氮中保存淋巴细胞的技术,该技术能使这些细胞在非常理想的条件下长时间存活。

相似文献

1
[Preservation of human lymphocytes in liquid nitrogen for the purpose of organ transplantation].[为器官移植目的将人淋巴细胞保存在液氮中]
Rev Chir Oncol Radiol O R L Oftalmol Stomatol Chir. 1976 Mar-Apr;25(2):133-6.
2
[Storage of the skin in liquid nitrogen and its experimental use].[皮肤在液氮中的储存及其实验应用]
Zhonghua Yi Xue Za Zhi. 1982 Sep;62(9):559-60.
3
Study of granulocyte-macrophage progenitor (CFUc) preservation after slow freezing of bone marrow in the gas phase of liquid nitrogen.
Exp Hematol. 1982 Apr;10(4):360-6.
4
Comparison of the HL-A phenotypes of lymphocytes and kidney cells determined by the fluorochromasia cytotoxicity assay.通过荧光色素细胞毒性试验对淋巴细胞和肾细胞的HL-A表型进行比较。
J Clin Invest. 1971 Feb;50(2):274-81. doi: 10.1172/JCI106492.
5
Long-term storage in liquid nitrogen does not affect cell viability in cardiac valve allografts.在液氮中进行长期储存不会影响心脏瓣膜同种异体移植物的细胞活力。
Cryobiology. 2008 Oct;57(2):113-21. doi: 10.1016/j.cryobiol.2008.07.008. Epub 2008 Jul 26.
6
Pretransplantation treatment of human teeth in tissue culture combined with storage in liquid nitrogen. A scanning electron microscopic study.组织培养结合液氮保存对人牙进行移植前处理:扫描电子显微镜研究
Exp Pathol. 1981;20(2):128-32.
7
[Sheep rosettes formed by lymphocytes preserved by freezing in liquid nitrogen].[通过在液氮中冷冻保存的淋巴细胞形成的绵羊红细胞玫瑰花结]
Nouv Presse Med. 1973 Nov 10;2(40):2689.
8
[Organization of a skin bank based on cryopreservation in liquid nitrogen. 1st results].
Z Med Lab Diagn. 1984;25(3):169-72.
9
Transport and storage of human teeth in vitro for autotransplantation and replantation.
J Oral Surg. 1976 Jan;34(1):13-8.
10
[Growth in vitro of human bone marrow after freezing in liquid nitrogen: analysis of a case].[液氮冷冻后人类骨髓的体外生长:一例分析]
Boll Soc Ital Biol Sper. 1980 Jan 15;56(1):62-8.