Pfragner R, Sadjak A, Eskici A, Walser V
Exp Pathol. 1981;20(2):128-32.
A combined method of tissue culture and storage in liquid nitrogen is described, which is suitable for covering the root surface of transplantable teeth with a dense cell layer: after an initial period of 10 days in tissue culture the teeth are stored in liquid nitrogen. 10 days before a transplantation the teeth are thawed and cultured again for 10 days. By this pretransplantation treatment a densely grown periodontal cell layer as well as an unlimited storage time are achieved.
描述了一种组织培养和液氮保存相结合的方法，该方法适用于用致密细胞层覆盖可移植牙齿的根面：在组织培养10天的初始阶段后，将牙齿保存在液氮中。在移植前10天，将牙齿解冻并再次培养10天。通过这种移植前处理，可以获得生长密集的牙周细胞层以及无限的保存时间。
