Stellwagen N C
Department of Biochemistry, University of Iowa, Iowa City 52242.
Electrophoresis. 1992 Sep-Oct;13(9-10):601-3. doi: 10.1002/elps.11501301121.
Previous studies have shown that the apparent pore size of agarose gels is dependent on the buffer in which the gel is cast and run (D.L. Holmes and N.C. Stellwagen, Electrophoresis 1990, 11, 5-15; N.C. Stellwagen and D.L. Holmes, Electrophoresis 1990, 11, 649-652). However, these studies, based on the mobility of DNA restriction fragments, neglected the effect of electroendosmosis. By measuring the mobility of vitamin B12 under various experimental conditions, it is shown here that electroendosmosis is highly buffer-dependent. When the observed mobilities of DNA are corrected for electroendomosis, the apparent pore radii of agarose gels are found to be independent of the casting buffer.
先前的研究表明，琼脂糖凝胶的表观孔径取决于凝胶制备和运行时所用的缓冲液（D.L. 霍姆斯和N.C. 斯特尔瓦根，《电泳》，1990年，第11卷，第5 - 15页；N.C. 斯特尔瓦根和D.L. 霍姆斯，《电泳》，1990年，第11卷，第649 - 652页）。然而，这些基于DNA限制性片段迁移率的研究忽略了电渗作用的影响。通过测量不同实验条件下维生素B12的迁移率，本文表明电渗作用高度依赖于缓冲液。当对DNA的观测迁移率进行电渗校正后，发现琼脂糖凝胶的表观孔半径与制备缓冲液无关。
