使用利尿剂治疗和未治疗的原发性轻度高血压患者（EHP）尿液中的血管紧张素转换酶：部分纯化与特性分析
Angiotensin converting enzymes from urine of treated and untreated essential mild hypertensive patients (EHP) with diuretic: partial purification and characterization.
作者信息
Alves K B, Casarini D E, da Costa R H, Plavnik F L, Portela J E, Marson O
机构信息
Department of Biochemistry, Escola Paulista de Medicina, SP, Brasil.
出版信息
Agents Actions Suppl. 1992;38 ( Pt 3):270-7.
PMID:1334355
Abstract
Urine of untreated EHP was eluted, on a ion-exchange chromatography, in two protein peaks with ACE activity, at 0.7 mS (BI) and 1.25 mS (BII), while urine of treated EHP, was eluted only in one peak with ACE activity (0.7 mS). BI (Mr, 88 kDa) and BII (Mr, 61 kDa) convert AI to AII, hydrolyze bradikinin, are inhibited by captopril, EDTA and metal ions.
摘要
未经处理的EHP尿液在离子交换色谱上洗脱时，出现两个具有ACE活性的蛋白峰，分别在0.7 mS（BI）和1.25 mS（BII）处，而经处理的EHP尿液仅在一个具有ACE活性的峰（0.7 mS）处洗脱。BI（分子量88 kDa）和BII（分子量61 kDa）将AI转化为AII，水解缓激肽，被卡托普利、EDTA和金属离子抑制。
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