革兰氏阴性菌大肠杆菌K-12中一种胺氧化酶里铜和3,4,6-三羟基苯丙氨酸醌辅因子的证据。
Evidence for copper and 3,4,6-trihydroxyphenylalanine quinone cofactors in an amine oxidase from the gram-negative bacterium Escherichia coli K-12.
作者信息
Cooper R A, Knowles P F, Brown D E, McGuirl M A, Dooley D M
机构信息
Bichemistry Department, University of Leicester, U.K.
出版信息
Biochem J. 1992 Dec 1;288 ( Pt 2)(Pt 2):337-40. doi: 10.1042/bj2880337.
Abstract
The cofactors present in a amine oxidase induced in Escherichia coli K-12 by growth on 2-phenylethylamine have been studied by spectroscopic methods. E.s.r. spectroscopy establishes the presence of cupric copper while resonance Raman spectroscopy on the phenylhydrazine derivative of the enzyme provides strong evidence for the oxidized form of 3,4,6-trihydroxyphenylalanine (TOPA) quinone. The amine oxidase should accordingly be classified as EC 1.4.3.6. This is the first report of such an amine oxidase in a Gram-negative bacterium.
摘要
通过光谱法研究了在2-苯乙胺上生长的大肠杆菌K-12中诱导产生的胺氧化酶中存在的辅因子。电子自旋共振光谱确定了铜离子的存在，而对该酶的苯肼衍生物进行的共振拉曼光谱为3,4,6-三羟基苯丙氨酸（TOPA）醌的氧化形式提供了有力证据。因此，该胺氧化酶应归类为EC 1.4.3.6。这是革兰氏阴性细菌中此类胺氧化酶的首次报道。
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