Zheng Z M, Specter S, Friedman H
Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa 33612.
Int J Immunopharmacol. 1992 Nov;14(8):1445-52. doi: 10.1016/0192-0561(92)90017-f.
Suppression by delta-9-tetrahydrocannabinol (THC) of tumor necrosis factor (TNF) production by macrophages has not been reported previously. The present study evaluated the effect in vitro of THC on soluble TNF-alpha production by cultured murine peritoneal macrophages. THC at 5 or 10 micrograms/ml added to medium [RPMI 1640 containing 10 ng LPS/ml, mouse IFN-gamma (100 u/ml), and 0.5% bovine serum albumin (BSA)] used to induce TNF significantly decreased TNF-alpha production by BALB/c mouse macrophages. Macrophages pretreated with THC at 0.1, 0.5, or 1.0 micrograms/ml in protein-free medium for 3 h at 37 degrees C, prior to TNF induction, also showed a decreased ability to produce TNF-alpha in a dose-dependent manner. Increasing the protein concentration from 0.5 to 5% BSA in the medium which was used to induce TNF prevented the inhibitory activity of THC. Human peripheral blood adherent cells treated with THC-containing medium produced less TNF-alpha than controls that were not exposed to THC. Thus, our data provide evidence that THC can inhibit TNF production by mouse and human macrophages. The drug's activity is concentration dependent and is related to the amount of serum protein in the medium used to induce this cytokine.
此前尚未有关于δ-9-四氢大麻酚（THC）对巨噬细胞肿瘤坏死因子（TNF）产生的抑制作用的报道。本研究评估了THC在体外对培养的小鼠腹腔巨噬细胞可溶性TNF-α产生的影响。将5或10微克/毫升的THC添加到用于诱导TNF的培养基[含有10纳克/毫升脂多糖、小鼠干扰素-γ（100单位/毫升）和0.5%牛血清白蛋白（BSA）的RPMI 1640]中，可显著降低BALB/c小鼠巨噬细胞的TNF-α产生。在TNF诱导前，将巨噬细胞在无蛋白培养基中用0.1、0.5或1.0微克/毫升的THC在37℃预处理3小时，也显示出以剂量依赖方式产生TNF-α的能力下降。在用于诱导TNF的培养基中将蛋白浓度从0.5%提高到5%的BSA可阻止THC的抑制活性。用含THC的培养基处理的人外周血贴壁细胞产生的TNF-α比未接触THC的对照细胞少。因此，我们的数据提供了证据表明THC可抑制小鼠和人巨噬细胞产生TNF。该药物的活性是浓度依赖性的，并且与用于诱导这种细胞因子的培养基中的血清蛋白量有关。
