Mandelkow E M, Drewes G, Biernat J, Gustke N, Van Lint J, Vandenheede J R, Mandelkow E
Max-Planck-Unit for Structural Molecular Biology, DESY, Hamburg, Germany.
FEBS Lett. 1992 Dec 21;314(3):315-21. doi: 10.1016/0014-5793(92)81496-9.
The Alzheimer-like state of tau protein includes phosphorylation by a proline-directed Ser/Thr kinase present in normal or pathological human brain. Extending earlier results on MAP kinase, we show here that the proline-directed kinase, GSK3, can induce an Alzheimer-like immune response involving several distinct and phosphorylatable epitopes at Ser-Pro motifs, as well as a gel mobility shift, similar to MAP kinase. Both kinases behave like microtubule-associated proteins in that they co-purify through cycles of assembly and disassembly, and both kinases are directly associated with paired helical filaments.
tau蛋白的阿尔茨海默样状态包括由正常或病理性人脑内存在的脯氨酸定向丝氨酸/苏氨酸激酶进行磷酸化。扩展早期关于丝裂原活化蛋白激酶（MAP激酶）的研究结果，我们在此表明，脯氨酸定向激酶GSK3可诱导一种阿尔茨海默样免疫反应，该反应涉及丝氨酸-脯氨酸基序处几个不同的可磷酸化表位，以及与MAP激酶类似的凝胶迁移率改变。这两种激酶在行为上类似于微管相关蛋白，因为它们通过组装和拆卸循环进行共纯化，并且这两种激酶都与双螺旋丝直接相关。
