毛翅抑制子突变对黑腹果蝇中由吉普赛诱导的叉毛和缺刻等位基因的显性效应。
Dominant effects of suppressor of Hairy-wing mutations on gypsy-induced alleles of forked and cut in Drosophila melanogaster.
作者信息
Hoover K K, Gerasimova T I, Chien A J, Corces V G
机构信息
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.
出版信息
Genetics. 1992 Nov;132(3):691-7. doi: 10.1093/genetics/132.3.691.
Abstract
Mutations induced by the gypsy retrotransposon in the forked (f) and cut (ct) loci render their expression under the control of the suppressor of Hairy-wing [su(Hw)] gene. This action is usually recessive, but su(Hw) acts as a dominant on the alleles fk, ctk and ctMRpN30. Molecular analysis of the gypsy element present in fk indicates that this allele is caused by the insertion of a modified gypsy in which the region normally containing twelve copies of the octamer-like repeat that interacts with the su(Hw) product is altered. Analysis of the gypsy element responsible for the ctk and ctMRpN30 mutations also reveals a correlation between the dominant action of su(Hw) and disruption of the octamer region. We propose that these disruptions alter the affinity and interaction of su(Hw) protein with gypsy DNA, thereby sensitizing the mutant phenotype to fluctuations in su(Hw) product.
摘要
由吉普赛逆转座子在叉状（f）和切割（ct）位点诱导的突变，使其表达受毛翅抑制因子[su（Hw）]基因的控制。这种作用通常是隐性的，但su（Hw）对fk、ctk和ctMRpN30等位基因起显性作用。对fk中存在的吉普赛元件的分子分析表明，该等位基因是由一个修饰的吉普赛插入引起的，其中通常包含十二个与su（Hw）产物相互作用的八聚体样重复序列的区域发生了改变。对导致ctk和ctMRpN30突变的吉普赛元件的分析也揭示了su（Hw）的显性作用与八聚体区域破坏之间的相关性。我们提出，这些破坏改变了su（Hw）蛋白与吉普赛DNA的亲和力和相互作用，从而使突变表型对su（Hw）产物的波动敏感。
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