Wohlsein P, Klimke A, Matschullat G, Röder B, Rim B M, Pohlenz J
Institut für Pathologie, Tierärztliche Hochschule, Hannover.
Berl Munch Tierarztl Wochenschr. 1992 Nov 1;105(11):366-72.
With regard to the legal regulations for the diagnosis of Aujeszky's disease in pigs, a retrospective immunoenzymatic study was performed on brains of 20 pigs, in which Aujeszky's disease had been diagnosed based only on clinical, macroscopical and histological findings, although the etiological agent could not be demonstrated neither by immunofluorescent technique nor by virological cultivation. Applying the peroxidase-antiperoxidase-(PAP) method, in 6 of 20 animals (30%) viral antigen of porcine herpesvirus type 1 was demonstrated in situ in at least two of five different locations of the brain. Reasons for negative results in immunohistological and virological investigations are discussed and the significance of the performed immunoenzymatic technique is pointed out.
关于猪奥耶斯基氏病诊断的法律法规，对20头猪的大脑进行了一项回顾性免疫酶研究。这些猪仅根据临床、大体和组织学检查结果被诊断为奥耶斯基氏病，尽管通过免疫荧光技术和病毒培养均未证实病原体。应用过氧化物酶-抗过氧化物酶（PAP）方法，在20只动物中的6只（30%）大脑的五个不同位置中至少有两个位置原位检测到了1型猪疱疹病毒的病毒抗原。讨论了免疫组织学和病毒学检查结果为阴性的原因，并指出了所采用免疫酶技术的意义。
