通过免疫荧光法在胰蛋白酶消化的石蜡包埋组织切片中检测伪狂犬病病毒抗原
Demonstration of Aujeszky's disease virus antigen in trypsin-digested paraffin-embedded tissue sections by immunofluorescence.
作者信息
Narita M, Inui S, Kobayashi M, Fukusho A
出版信息
Natl Inst Anim Health Q (Tokyo). 1983 Fall;23(3):105-7.
PMID:6330574
Abstract
After trypsin digestion of buffered formalin-fixed, paraffin-embedded brain section, immunofluorescent indication of Aujeszky's disease virus antigen was successful in experimentally infected cow and goat. This result was comparable to those obtained with light and electron microscopic examinations. This method will be diagnostically useful where fresh-frozen tissue is not available.
摘要
对经缓冲福尔马林固定、石蜡包埋的脑切片进行胰蛋白酶消化后，在实验感染的牛和山羊中成功实现了奥耶斯基病病毒抗原的免疫荧光显示。该结果与通过光学显微镜和电子显微镜检查获得的结果相当。在无法获得新鲜冷冻组织的情况下，该方法将具有诊断用途。
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