HeLa细胞中磷脂酰胆碱的基础周转率和佛波酯刺激的周转率涉及不同途径。
Basal and phorbol-ester-stimulated turnover of phosphatidylcholine in HeLa cells involve different pathways.
作者信息
Hii C S, Edwards Y S, Murray A W
机构信息
School of Biological Sciences, Flinders University, Adelaide, South Australia, Australia.
出版信息
Biochem J. 1992 Dec 15;288 ( Pt 3)(Pt 3):983-5. doi: 10.1042/bj2880983.
Abstract
HeLa cells prelabelled with [3H]lysoplatelet-activating factor (lyso-PAF) accumulated phosphatidic acid (PtdOH) when incubated in the presence of either propranolol (an inhibitor of PtdOH phosphohydrolase) or phorbol ester. In the presence of ethanol, phorbol ester but not propranolol stimulated the accumulation of phosphatidylethanol, an index of phospholipase D activity. Incubation of cells with [3H]lyso-PAF led to a rapid accumulation of label in diacylglycerol (DG) followed by a delayed accumulation in PtdOH. It is concluded that propranolol-induced PtdOH accumulation is derived from DG by DG kinase and does not involve phospholipase D.
摘要
预先用[3H]溶血血小板活化因子（lyso-PAF）标记的HeLa细胞，在普萘洛尔（一种磷脂酸磷酸水解酶抑制剂）或佛波酯存在的情况下孵育时会积累磷脂酸（PtdOH）。在乙醇存在的情况下，佛波酯而非普萘洛尔刺激了磷脂酰乙醇的积累，磷脂酰乙醇是磷脂酶D活性的一个指标。用[3H]lyso-PAF孵育细胞会导致二酰基甘油（DG）中标记物迅速积累，随后PtdOH中出现延迟积累。得出的结论是，普萘洛尔诱导的PtdOH积累是由DG通过DG激酶产生的，并不涉及磷脂酶D。
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