JAKUS M A
J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):243-52. doi: 10.1083/jcb.2.4.243.
Descemet's membrane, previously thought to be "structureless," has been found to be characterized by a fine structure of great regularity. Sections perpendicular to the plane of the membrane surface appeared to be cross-striated, with narrow dark bands separated by wide light bands traversed by fine filaments. Tangential sections showed a two-dimensional array of dark nodes and thin internodal filaments which connected each node with the six others around it to form a hexagonal figure. The average distance between nodes, and between the dark bands in transverse sections, was about 1070 A; the width of the nodes was about 270 A; and the width of the connecting filaments was less than 100 A. This pattern has been found in all species of Descemet's membrane so far examined, although it appeared to be better developed in some forms than in others. So far as is known, it has not been observed in any other type of tissue. The differentiation of Descemet's membrane has been followed in the chick embryo. It appeared late and developed slowly but showed the characteristic fine structure from the time it could be clearly identified. The evidence for placing Descemet's membrane in the collagen class of proteins is strongly supplemented by the results obtained from x-ray diffraction examination by Rougvie and Bear.
曾被认为是“无结构的”后弹力层，现已发现其具有高度规则的精细结构。垂直于膜表面平面的切片呈现出横纹状，狭窄的暗带被宽的亮带分隔，亮带中有细丝穿过。切向切片显示出由暗节点和细的节间细丝组成的二维阵列，这些细丝将每个节点与其周围的六个节点相连，形成六边形图案。节点之间以及横切面上暗带之间的平均距离约为1070埃；节点的宽度约为270埃；连接细丝的宽度小于100埃。到目前为止，在所有已检查的后弹力层物种中都发现了这种图案，尽管在某些形式中它比其他形式发育得更好。据所知，在任何其他类型的组织中都未观察到这种情况。在鸡胚中追踪了后弹力层的分化过程。它出现较晚且发育缓慢，但从能够清晰识别时起就显示出特征性的精细结构。Rougvie和Bear通过X射线衍射检查获得的结果有力地补充了将后弹力层归为胶原蛋白类蛋白质的证据。
