Siegenthaler G, Tomatis I, Didierjean L, Jaconi S, Saurat J H
Department of Dermatology, University Hospital, Geneva, Switzerland.
Dermatology. 1992;185(4):251-6. doi: 10.1159/000247462.
Cellular retinoic acid-binding proteins (CRABPs) might exert a physiological function by controlling the intracellular levels of free retinoic acid. The aim of this study was to analyze the relative expression of CRABP-I and CRABP-II in lesional (LPS) and nonlesional (NLPS) psoriatic skin. CRABP-I and -II proteins were analyzed by a PAGE-autoradioblotting technique, and their respective mRNA were studied by RNA blot and in situ hybridization. We found that CRABP-II levels were 6-fold higher in LPS and 2-fold in NLPS as compared to normal skin, whereas CRABP-I levels were decreased in NLPS and LPS. CRABP-II mRNA were grossly overexpressed in all LPS and some NLPS specimens. These results indicate a switch to the overexpression of CRABP-II mRNA in psoriasis which induces high levels of the protein mainly in LPS; these observations may be relevant to the pathophysiology and therapy of psoriasis as CRABP-I and -II have different ligand-binding affinities.
细胞视黄酸结合蛋白（CRABPs）可能通过控制细胞内游离视黄酸的水平发挥生理功能。本研究的目的是分析CRABP-I和CRABP-II在银屑病皮损（LPS）和非皮损（NLPS）皮肤中的相对表达。采用PAGE-放射自显影印迹技术分析CRABP-I和-II蛋白，通过RNA印迹和原位杂交研究它们各自的mRNA。我们发现，与正常皮肤相比，LPS中CRABP-II水平高6倍，NLPS中高2倍，而NLPS和LPS中CRABP-I水平降低。CRABP-II mRNA在所有LPS和一些NLPS标本中均明显过度表达。这些结果表明，银屑病中CRABP-II mRNA过度表达发生了转变，主要在LPS中诱导该蛋白的高水平表达；由于CRABP-I和-II具有不同的配体结合亲和力，这些观察结果可能与银屑病的病理生理学和治疗有关。
