Allen J W, Viel L, Bateman K G, Nagy E, Røsendal S, Shewen P E
Department of Clinical Studies, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1992 Oct;56(4):281-8.
Acute and convalescent serum samples were taken from 59 calves with signs of respiratory disease (cases) and 60 clinically normal animals (controls) during their first month in the feedlot. Sera were analyzed for antibodies to bovine parainfluenza 3 (PI3) virus by hemagglutination inhibition, to bovine viral diarrhea (BVD) virus, bovine respiratory syncytial (BRS) virus and bovine herpesvirus 1 (BHV1) by virus neutralization, and to Pasteurella haemolytica by indirect agglutination (PhIA) and cytotoxin neutralization (PhCN) tests. There was minimal evidence of serological activity to BHV1. Serological activity to the other agents occurred commonly and the prevalence of acute titers and their mean values was similar in case and control groups. Mean convalescent PI3 and P. haemolytica (PhIA) titers were higher in controls than cases (p < 0.01) but, otherwise, convalescent titers did not differ between groups. The incidence of seroconversion was similar in both groups for all agents except for PI3 virus which was more frequent in controls than cases (p < 0.0001). There was a positive association between PhIA and CN seroconversion and isolation of P. haemolytica from bronchoalveolar lavage (BAL) fluid (p < 0.1). The measure of agreement (kappa) between seroconversion with the P. haemolytica PhIA and PhCN tests was 0.51. Bacteriological and cytological evaluations of the respiratory tract were made using BAL. No associations were evident between serological titers and pulmonary cytology. A multivariate logistic analysis was used to evaluate associations between disease status and serological, bacteriological and cytological data. Cases were positively associated with the presence of neutrophils and Pasteurella multocida in BAL fluid and negatively associated with PI3 virus and PhIA seroconversion.
在育肥牛场的第一个月内，从59头有呼吸道疾病症状的犊牛（病例组）和60头临床正常的动物（对照组）采集急性和恢复期血清样本。通过血凝抑制试验分析血清中抗牛副流感3型（PI3）病毒的抗体，通过病毒中和试验分析抗牛病毒性腹泻（BVD）病毒、牛呼吸道合胞体（BRS）病毒和牛疱疹病毒1型（BHV1）的抗体，通过间接凝集试验（PhIA）和细胞毒素中和试验（PhCN）分析抗溶血巴斯德菌的抗体。几乎没有证据表明存在针对BHV1的血清学活性。针对其他病原体的血清学活性普遍存在，病例组和对照组中急性滴度的患病率及其平均值相似。对照组中PI3和溶血巴斯德菌（PhIA）的恢复期平均滴度高于病例组（p<0.01），但除此之外，两组之间的恢复期滴度没有差异。除PI3病毒外，两组中所有病原体的血清转化发生率相似，PI3病毒在对照组中的发生率高于病例组（p<0.0001）。PhIA和CN血清转化与从支气管肺泡灌洗（BAL）液中分离出溶血巴斯德菌之间存在正相关（p<0.1）。溶血巴斯德菌PhIA和PhCN试验的血清转化一致性测量值（kappa）为0.51。使用BAL对呼吸道进行细菌学和细胞学评估。血清学滴度与肺细胞学之间没有明显关联。使用多变量逻辑分析来评估疾病状态与血清学、细菌学和细胞学数据之间的关联。病例与BAL液中中性粒细胞和多杀性巴氏杆菌的存在呈正相关，与PI3病毒和PhIA血清转化呈负相关。
