Ye X Z
Chongqing Cancer Research Institute.
Zhonghua Bing Li Xue Za Zhi. 1992 Jun;21(3):149-51.
Immunohistochemical staining and histochemical approach for mucin were applied in studying 58 cases of colorectal adenomas. Among them, there were 13 cases of multiple adenomas (polyps), 5 cases of inflammatory polyps and 5 cases of juvenile polyps. The results indicated that positive expression of both McAb MC5 and Ulex europaeus agglutinin-1(UEA-1) were correlating with the degree of dysplasia, the histological type and the size of adenomas. The positive expression of peanut agglutinin (PNA) was correlating with the degree of dysplasia. Moderate and heavy stainings were mainly seen in adenomas accompanying with moderate and severe dysplasia, as well as adenomas with early carcinomatous changes. PAT-KOH-PAS method could sensitively reflect the occurrence of dysplasia and malignancy of the adenomas. These findings support the concept of an adenoma(dysplasia)-carcinoma sequence. In comparing multiple adenomas (number of adenomas > 100) with the solitary ones, a notable difference was obtained in the expression of McAb MC5. It's concluded that combined use of certain immunohistochemical and histochemical stainings is well be useful in detecting the malignant potentiality of adenoma.
采用免疫组织化学染色和黏蛋白组织化学方法对58例大肠腺瘤进行研究。其中，多发性腺瘤（息肉）13例，炎性息肉5例，幼年性息肉5例。结果显示，单克隆抗体MC5和荆豆凝集素-1（UEA-1）的阳性表达均与发育异常程度、组织学类型及腺瘤大小相关。花生凝集素（PNA）的阳性表达与发育异常程度相关。中度和重度染色主要见于伴有中度和重度发育异常的腺瘤以及有早期癌变改变的腺瘤。PAT-KOH-PAS法能敏感地反映腺瘤发育异常和恶变的发生情况。这些发现支持腺瘤（发育异常）-癌序列的概念。在比较多发性腺瘤（腺瘤数量>100）和单发腺瘤时，单克隆抗体MC5的表达存在显著差异。结论是，联合使用某些免疫组织化学和组织化学染色有助于检测腺瘤的恶性潜能。
