Workman T, Shen D, Woodard L, Yilma T
Am J Vet Res. 1986 Jul;47(7):1507-12.
An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect bovine antibody to vesicular stomatitis virus (VSV). Serum samples from cows experimentally infected with the New Jersey serotype of VSV (VSV-NJ) were assayed by the ELISA and serum-neutralization (SN) assay. The ELISA was as sensitive as the SN assay in detecting bovine antibody to VSV. The correlation between SN titers and ELISA values at absorbance at 405 nm was statistically significant. The ELISA was not specific for VSV-NJ, however, and could detect serum samples positive to the Indiana serotype of VSV that had SN titers of greater than or equal to 480. Nonspecific reactions were due to cross-reactive group-specific viral proteins that are shared by both serotypes. The cross-reactivity allows the use of a single rapid test in identifying both serotypes of VSV from the other exotic vesicular diseases, especially foot-and-mouth disease. The ELISA titers of serum samples positive for VSV-NJ were comparable with the corresponding SN titers of each sample. The sensitivity, rapidity, and ease of the ELISA system and the use of a single test in identifying both serotypes of VSV from the other exotic vesicular diseases make this ELISA suitable as a rapid diagnostic assay for VS.
开发了一种间接酶联免疫吸附测定（ELISA）来检测牛抗水疱性口炎病毒（VSV）抗体。用ELISA和血清中和（SN）测定法检测了经新泽西血清型VSV（VSV-NJ）实验感染的奶牛的血清样本。ELISA在检测牛抗VSV抗体方面与SN测定法一样灵敏。SN滴度与405nm吸光度下的ELISA值之间的相关性具有统计学意义。然而，ELISA对VSV-NJ不具有特异性，并且可以检测对VSV印第安纳血清型呈阳性的血清样本，其SN滴度大于或等于480。非特异性反应是由于两种血清型共有的交叉反应性组特异性病毒蛋白引起的。这种交叉反应性使得可以使用单一快速检测方法从其他外来水疱性疾病，特别是口蹄疫中鉴定出VSV的两种血清型。VSV-NJ阳性血清样本的ELISA滴度与每个样本相应的SN滴度相当。ELISA系统的敏感性、快速性和简便性以及使用单一检测方法从其他外来水疱性疾病中鉴定出VSV的两种血清型，使得这种ELISA适合作为VS的快速诊断测定方法。
