Frank J, Radermacher M
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Ultramicroscopy. 1992 Oct;46(1-4):241-62. doi: 10.1016/0304-3991(92)90018-f.
The random-conical reconstruction method has been highly successful in three-dimensional imaging of macromolecules under low-dose conditions. This article summarizes the different steps of this technique as applied to molecules prepared with negative staining or vitreous ice, and sketches out the current directions of development. We anticipate that by using new instrumental developments, transfer function correction and computational refinement techniques, a resolution in the range of 7-10 A could ultimately be achieved.
随机圆锥重建方法在低剂量条件下对大分子进行三维成像方面取得了巨大成功。本文总结了该技术应用于负染色或玻璃态冰制备的分子时的不同步骤，并概述了当前的发展方向。我们预计，通过使用新的仪器发展、传递函数校正和计算细化技术，最终可实现7至10埃范围内的分辨率。
