Chiu W, Schmid M F, Prasad B V
Werna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Biophys J. 1993 May;64(5):1610-25. doi: 10.1016/S0006-3495(93)81533-9.
Electron microscopic analysis can be used to determine the three-dimensional structures of macromolecules at resolutions ranging between 3 and 30 A. It differs from nuclear magnetic resonance spectroscopy or x-ray crystallography in that it allows an object's Coulomb potential functions to be determined directly from images and can be used to study relatively complex macromolecular assemblies in a crystalline or noncrystalline state. Electron imaging already has provided valuable structural information about various biological systems, including membrane proteins, protein-nucleic acid complexes, contractile and motile protein assemblies, viruses, and transport complexes for ions or macromolecules. This article, organized as a series of lectures, presents the biophysical principles of three-dimensional analysis of objects possessing different symmetries.
电子显微镜分析可用于在3至30埃的分辨率范围内确定大分子的三维结构。它与核磁共振光谱或X射线晶体学不同，因为它允许直接从图像中确定物体的库仑势函数，并且可用于研究处于结晶或非晶态的相对复杂的大分子组装体。电子成像已经提供了有关各种生物系统的有价值的结构信息，包括膜蛋白、蛋白质-核酸复合物、收缩和运动蛋白组装体、病毒以及离子或大分子的转运复合物。本文以一系列讲座的形式呈现了对具有不同对称性的物体进行三维分析的生物物理原理。
