Voropaeva S D, Vartanian Zh S, Ankirskaia A S
Antibiotiki. 1976 Jun;21(6):527-30.
Transduction of extrachromosomal markers of resistance to penicillin and erythromycin in staphylococcal strains isolated from patients was studied. Two transduction methods were compared, i. e. transduction with a phage filtrate of the donor culture resistant to erythromycin and transduction on mixed cultivation of the donor and recipient. A higher transduction rate was observed with the latter method. Mixed cultivation of the donor cultures resistant to penicillin or erythromycin and the recipient strains of the wild type sensitive to these antibiotic resulted in transduction of the respective markers. Transductants which acquired prophage 6 simultaneously with marker Egg became donors of erythromycin resistance.
对从患者分离出的葡萄球菌菌株中青霉素和红霉素抗性的染色体外标记进行了转导研究。比较了两种转导方法,即使用对红霉素耐药的供体培养物的噬菌体滤液进行转导以及供体与受体混合培养时的转导。观察到后一种方法的转导率更高。对青霉素或红霉素耐药的供体培养物与对这些抗生素敏感的野生型受体菌株混合培养导致了各自标记的转导。同时获得原噬菌体6和标记Egg的转导子成为红霉素抗性的供体。