Lantos J
Acta Microbiol Acad Sci Hung. 1977;24(2):107-13.
In the course of an outbreak of enteritis and conjunctivitis, Staphylococcus aureus was isolated from newborn infants. Strains cultured at a later phase of the outbreak differed from those found at the beginning in being resistant to several antibiotics, showing resistance to typing phages and releasing phages of the same lysis spectrum (10(9) p.f.u./ml after heating at 56 degrees C for 2 min). Transduction experiments with a strain and its cell-free lysate showed that inducible erythromycin resistance was transferable to strains isolated at the beginning of the outbreak and to laboratory strains. Plasmid origin of resistance was confirmed by (i) high transduction frequency; (ii) transduction to RN981 rec- mutants; (iii) kinetics of transduction; (iv) elimination of resistance. Mixed culture experiments yielded transductants at high frequency with resistance to erythromycin, streptomycin and tetracycline.
在一次肠炎和结膜炎暴发期间，从新生儿中分离出了金黄色葡萄球菌。在暴发后期培养的菌株与开始时发现的菌株不同，对几种抗生素具有抗性，对分型噬菌体表现出抗性，并释放出具有相同裂解谱的噬菌体（在56℃加热2分钟后为10⁹噬菌斑形成单位/毫升）。用一株菌株及其无细胞裂解物进行的转导实验表明，可诱导的红霉素抗性可转移到暴发开始时分离的菌株和实验室菌株。通过以下几点证实了抗性的质粒起源：（i）高转导频率；（ii）转导至RN981 rec-突变体；（iii）转导动力学；（iv）抗性消除。混合培养实验产生了对红霉素、链霉素和四环素具有抗性的高频转导子。
