3',5'-环磷酸腺苷：肾上腺素在成年大鼠肝细胞原代培养物中对氨基酸转运的独立诱导作用
3':5'-cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells.
作者信息
Pariza M W, Butcher F R, Becker J E, Potter V R
出版信息
Proc Natl Acad Sci U S A. 1977 Jan;74(1):234-7. doi: 10.1073/pnas.74.1.234.
Liver cells were obtained from adult rats by a collagenase perfusion technique and cultured as monolayers in serum-free media. Epinephrine and isoproterenol both induced large increases in intracellular adenosine 3':5'-monophosphate (cAMP) within 1-2 min whereas epinephrine (but not isoproterenol) induced 2- to 3-fold increases in the rate of alpha-aminoisobutyric acid transport within 2-4 hr after a 1 hr lag. Propranolol abolished the increase in cAMP elicited by epinephrine and isoproterenol, but did not block the induction of alpha-aminoisobutyric acid transport by epinephrine. In contrast, dihydroergotamine abolished and phentolamine diminished the induction of alpha-aminoisobutyric acid transport by epinephrine but did not decrease the stimulation of cAMP levels by epinephrine. Epinephrine dose response curves for cAMP and alpha-aminoisobutyric acid transport were similar. Once exposed to epinephrine, cells became refractory to further stimulation of cAMP levels by epinephrine.
采用胶原酶灌注技术从成年大鼠获取肝细胞，并在无血清培养基中单层培养。肾上腺素和异丙肾上腺素均可在1至2分钟内使细胞内3':5'-单磷酸腺苷（cAMP）大幅增加，而肾上腺素（而非异丙肾上腺素）在1小时延迟后2至4小时内可使α-氨基异丁酸转运速率提高2至3倍。普萘洛尔可消除肾上腺素和异丙肾上腺素引起的cAMP增加，但不阻断肾上腺素诱导的α-氨基异丁酸转运。相反，双氢麦角胺可消除肾上腺素诱导的α-氨基异丁酸转运，酚妥拉明可使其减弱，但二者均不降低肾上腺素对cAMP水平的刺激。肾上腺素对cAMP和α-氨基异丁酸转运的剂量反应曲线相似。一旦暴露于肾上腺素，细胞对肾上腺素进一步刺激cAMP水平变得不敏感。
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