Gray B N, Watkins E
Aust J Exp Biol Med Sci. 1976 Feb;54(1):57-70. doi: 10.1038/icb.1976.6.
Pancreatic islets have been isolated from the exocrine pancreas of inbred rats by the collagenase digestion method. Transplantation of isolated islets into the portal venous system of streptozotocin diabetic recipients resulted in complete abrogation of the diabetic state as measured by non-fasting serum glucose level, 24 h urinary output, rate of weight gain and glucose tolerance test. Transplantation to other sites resulted in less than optimal survival and function of islets. Allogeneic islets, transplanted across weak histocompatibility barriers, can survive and function for prolonged periods of time when transplanted recipients are immunosuppressed with antilymphocyte serum (ALS). Recipients of allogeneic islets, after a period of immunosuppression with ALS, become permanently tolerant to the allografted islets and to subsequent skin grafts from similar allogeneic donors. Allografted islets are able to prevent the occurrence of diabetic renal and ophthalmic changes that occur in control diabetic animals which had not undergone transplantation.
通过胶原酶消化法从近交系大鼠的外分泌胰腺中分离出胰岛。将分离的胰岛移植到链脲佐菌素诱导的糖尿病受体的门静脉系统中，通过非空腹血清葡萄糖水平、24小时尿量、体重增加率和葡萄糖耐量试验测量，结果显示糖尿病状态完全消除。移植到其他部位则导致胰岛的存活和功能不尽人意。当移植受体用抗淋巴细胞血清（ALS）进行免疫抑制时，跨越弱组织相容性屏障移植的同种异体胰岛可以长期存活并发挥功能。在用ALS进行一段时间的免疫抑制后，同种异体胰岛的受体对移植的同种异体胰岛以及来自相似同种异体供体的后续皮肤移植产生永久性耐受。移植的同种异体胰岛能够预防未接受移植的对照糖尿病动物中发生的糖尿病性肾脏和眼部病变。
