Finch D R, Wise P H, Morris P J
Diabetologia. 1977 May;13(3):195-9. doi: 10.1007/BF01219699.
Adult rat islets harvested by the collagenase digestion/Ficoll separation technique were injected into the splenic pulp in 9 syngeneic (Lewis-leads to Lewis and WaG leads to WaG) and 13 allogeneic [(DA X Lewis) F1 leads to Lewis] experiments. Normal serum glucose levels and 24 hour urine volumes were restored in all 9 syngeneic recipients and in 11 out of the 13 allogeneic recipients in a mean of 3.3 days. Splenectomy performed on 3 of the 9 syngeneic recipients 110-178 days after transplantation resulted in a prompt return to the diabetic state. In all the remaining syngeneic recipients, normal values have persisted for the current period of observation of 6 months. In 5 untreated allogeneic recipients, rejection occurred in a mean of 5.2 days. The administration of a short course of ALS (1 ml I.P. days-1, 1, 3 and 5) to the remaining 6 animals greatly prolonged graft survival with all animals remaining normoglycaemic for at lest 4 weeks. These results were not significantly different from those recorded in comparable groups of intra-portal allogeneic islet recipients.
采用胶原酶消化/Ficoll分离技术获取成年大鼠胰岛，将其注入9只同基因（Lewis→Lewis和WaG→WaG）和13只异基因[（DA×Lewis）F1→Lewis]实验动物的脾髓中。所有9只同基因受体以及13只异基因受体中的11只，平均在3.3天内恢复了正常血糖水平和24小时尿量。在移植后110 - 178天，对9只同基因受体中的3只进行脾切除，结果迅速恢复到糖尿病状态。在其余所有同基因受体中，在目前6个月的观察期内，血糖值一直保持正常。在5只未治疗的异基因受体中，平均在5.2天发生排斥反应。对其余6只动物短期给予抗淋巴细胞血清（腹腔注射1毫升，第1、3和5天），显著延长了移植物存活时间，所有动物至少4周保持血糖正常。这些结果与门静脉内异基因胰岛受体的相应组记录结果无显著差异。
