Wynne K N, Renwick A G
Biochem J. 1976 May 15;156(2):419-25. doi: 10.1042/bj1560419.
The chemical syntheses of the 3-sulphates of 16 alpha-acetoxy-, 16 alpha-hydroxy- and 16 beta-hydroxy-dehydroepiandrosterone as their triethylammonium salts are described preparatory to studies of the metabolism of [7 alpha-3 H]dehydroepiandrosterone sulphate by homogenates of human foetal liver. Five main radioactive products of the incubation were separated by partition chromatography on a Celite column. Two were identified as 16 alpha-and 16 beta-hydroxydehydroepiandrosterone 3-sulphates by crystallization to constant specific radioactivity before and after solvolysis. The yields of the conversion to the two epimers were 24.4% (16 alpha) and 1.8% (16 beta).
为了研究[7α-³H]硫酸脱氢表雄酮在人胎儿肝脏匀浆中的代谢情况，本文描述了16α-乙酰氧基-、16α-羟基-和16β-羟基-脱氢表雄酮的3-硫酸盐作为其三乙铵盐的化学合成方法。通过在硅藻土柱上进行分配色谱法分离出孵育的五种主要放射性产物。其中两种通过在溶剂解前后结晶至恒定比放射性，被鉴定为16α-和16β-羟基脱氢表雄酮3-硫酸盐。转化为这两种差向异构体的产率分别为24.4%（16α）和1.8%（16β）。
