铜（Ⅱ）-抗坏血酸对红细胞膜脂质过氧化、镁（Ⅱ）-ATP酶活性和血影蛋白的影响以及促红细胞生成素的逆转作用

Effect of Cu(2+)-ascorbic acid on lipid peroxidation, Mg(2+)-ATPase activity and spectrin of RBC membrane and reversal by erythropoietin.

作者信息

Chattopadhyay A, Das Choudhury T D, Basu M K, Datta A G

机构信息

Guha Institute of Biochemistry, Calcutta, India.

出版信息

Mol Cell Biochem. 1992 Dec 2;118(1):23-30. doi: 10.1007/BF00249691.

DOI:10.1007/BF00249691

PMID:1336813

Abstract

The effect of erythropoietin (Ep), a glycoprotein hormone, has been studied on lipid peroxidation induced by Cu2+ and ascorbate in vitro, Mg2+ ATPase activity and spectrin of RBC membrane. Our present investigation reveals that Cu2+ and ascorbic acid increases lipid peroxidation of RBC membrane significantly. It has further been observed that under the same experimental condition spectrin, a major cytoskeleton membrane protein, and Mg(2+)-ATPase activity of RBC membrane decrease significantly. However, exogenous administration of Ep completely restores lipid peroxidation and Mg(2+)-ATPase activity and partially recovers spectrin of RBC membrane.

摘要

促红细胞生成素（Ep）是一种糖蛋白激素，其对体外由铜离子（Cu2+）和抗坏血酸诱导的脂质过氧化作用、红细胞膜的镁离子（Mg2+）ATP酶活性及血影蛋白的影响已得到研究。我们目前的研究表明，Cu2+和抗坏血酸显著增加红细胞膜的脂质过氧化作用。进一步观察发现，在相同实验条件下，红细胞膜的主要细胞骨架膜蛋白血影蛋白及Mg(2+)-ATP酶活性显著降低。然而，外源性给予Ep可完全恢复脂质过氧化作用和Mg(2+)-ATP酶活性，并部分恢复红细胞膜的血影蛋白。