大肠杆菌转化体中形成的质粒DNA的异常结构。

Unusual structure of plasmid DNA formed in transformants of Escherichia coli.

作者信息

Kato M, Furuno A

机构信息

Department of Life Sciences, College of Integrated Arts and Sciences, University of Osaka Prefecture, Sakai, Japan.

出版信息

Res Microbiol. 1992 Sep;143(7):665-9. doi: 10.1016/0923-2508(92)90061-r.

DOI:10.1016/0923-2508(92)90061-r

PMID:1336880

Abstract

An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and the recA genotype may relate to accumulation of the plasmids conforming to the unusual topology.

摘要

在大肠杆菌菌株的转化子中观察到质粒pUC18的一种异常结构成分。该分子在琼脂糖凝胶电泳中的迁移速度比超螺旋拓扑异构体更快，并且难以用溴化乙锭染色。这种异常拓扑结构的出现与gyrase突变无关，recA基因型可能与符合异常拓扑结构的质粒积累有关。