用IS256对耐甲氧西林金黄色葡萄球菌进行分型

Typing of methicillin-resistant Staphylococcus aureus with IS256.

作者信息

Wei M Q, Udo E E, Grubb W B

机构信息

School of Biomedical Sciences, Curtin University of Technology, Bentley, W.A., Australia.

出版信息

FEMS Microbiol Lett. 1992 Dec 1;78(2-3):175-80. doi: 10.1016/0378-1097(92)90021-f.

DOI:10.1016/0378-1097(92)90021-f

PMID:1337051

Abstract

A simple one-step procedure is described for specifically amplifying and labelling insertion element IS256 which is associated with the gentamicin-resistance transposon Tn4001. The product has been used to probe DNA digests of methicillin-resistant Staphylococcus aureus. The resulting restriction fragment length polymorphisms were found to be able to distinguish isolates which were indistinguishable by other typing methods. The probe also hybridised with methicillin-resistant Staphylococcus aureus which were isolated before the emergence of gentamicin resistance, demonstrating its usefulness in typing species other than those that are gentamicin-resistant.

摘要

描述了一种简单的一步程序，用于特异性扩增和标记与庆大霉素抗性转座子Tn4001相关的插入元件IS256。该产物已用于探测耐甲氧西林金黄色葡萄球菌的DNA消化物。发现由此产生的限制性片段长度多态性能够区分用其他分型方法无法区分的分离株。该探针还与在庆大霉素抗性出现之前分离的耐甲氧西林金黄色葡萄球菌杂交，证明了其在对除耐庆大霉素菌株以外的其他菌株进行分型方面的有用性。